The calcium assay is a procedure in which a sample is treated with a solution containing a specific amount of calcium. This solution is typically prepared for research purposes and is not intended for diagnostic procedures or use in animals. If you’re planning to use this calcium assay to test calcium levels in your own body, you should be aware of the safety precautions to be taken.
What Is The Purpose?
BD’s Calcium Assay Kits detect intracellular calcium mobilization in live cells using an optimized dye. The dye undergoes an electronic change when bound to calcium, which provides greater fluorescence and less background. This method is ideal for high-throughput environments. To run the Calcium Assay, you should use optimized cell numbers to achieve maximum sensitivity and minimize the risk of background fluorescence. You should also prepare a confluent monolayer of cells to perform the assay.
What Are Genetically Encoded Calcium Indicators?
GECIs (genetically encoded calcium indicators) are highly sensitive and specific in a range of applications, including primary screening and drug development programs. GECIs have been optimized to compete with synthetic calcium indicators in several applications, including high-throughput screening. The stable expression of GECIs also minimizes the cost of reagents and simplifies the calcium assay. In a calcium assay, the calcium indicator is highly expressed in cells and can be used to monitor intracellular calcium mobilization and GPCR activity.
Increased Fluorescence Impact
PBX and neuropeptides peptides were synthesized by BiomerTech. Kalypsys supplied a 1,536-well tissue culture-treated black plate, and BD provided a calcium assay kit for the experiment. To test the cellular mobilization of calcium, Fluo-8 NW was used as a fluorescent dye. The fluorescence of the dye is directly related to the amount of intracellular calcium released. Using this technique, the enzyme anion transporter, an enzyme involved in calcium mobilization, is highly active in certain cells.
GCaMP6s and fluo-4 had similar working dynamic ranges, but their optimal fluorescence sensitivity was significantly decreased when compared to 293-F cells stained with fluo-4. The decrease in signal intensity did not have an impact on assay performance but may affect certain applications that require maximal light output. This increased fluorescence was due to labelling of intracellular organelles. However, the working dynamic range is smaller than optimal conditions.
What Is It Used For?
The calcium assay kit from BioAssay Systems is a simple and accurate procedure for measuring calcium in biological samples. The procedure is based on the principle of Ca2+ sequestration, which is responsible for many cellular processes. The calcium concentration in blood is tightly regulated, so variations in serum levels may have devastating consequences. Calcium affects skeletal musculature and heart function and has significant roles in blood clotting. Calcium is bound to several proteins in plasma.
